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Image Search Results
Journal: BMC Plant Biology
Article Title: Large-scale proteomic analysis of the grapevine leaf apoplastic fluid reveals mainly stress-related proteins and cell wall modifying enzymes
doi: 10.1186/1471-2229-13-24
Figure Lengend Snippet: 2-DE Coomassie blue stained gels from whole leaf proteome (A) and apoplastic fluid (B) of V. vinifera leaves . Protein extracts were separated on 18 cm, pH 3–10 non-linear gradient IPG gels (IEF) followed by 12% SDS PAGE. Arrows show the position of the analyzed spots. The protein spot number refers to Additional file 1 for whole leaf proteins and to Additional file 2 for apoplastic proteins. White arrows show examples of common proteins found in gel B (RuBisCo subunit (09), ATPase (90) and sedoheptulose-1,7 bisphosphatase (52)) and their respective spot in gel A.
Article Snippet: For analytical 2D-PAGE separations, samples containing 250 μg proteins in rehydration buffer were applied on
Techniques: Staining, SDS Page